Figure 1
From: Bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma
Representative pictures (10×) of BoHV-4-EFGPΔTK infected F98 (A), GLI36 (C) and GL261 (E) cells at 96 hours (hs) post infection (P.I.), visualized by phase contrast (PC) fluorescence with a FITC filter for EGFP expression or with DAPI filter for nuclear counterstaining (bar = 100 μm). The respective titers (expressed as log10 of Tissue Cells Infectious Dose/50 [TCID50] per ml-1) of viral particles released during the time at 24 and 96 hours (hs) post infection (P.I.) are shown in B, D and F. Values are the mean ± standard error of three independent experiments. (G) GL261 mouse glioblastoma cell line (a, bar = 25 μm), F98 rat glioma cell line (b, bar = 25 μm) and GLI36 human glioma cell line (c, bar = 10 μm) infected with BoHV-4EGFPΔTK for 72 hours. CPE induced by infection shows a prevalence of necrosis (ANOVA, ** p < 0.001, *p < 0.05).