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Table 4 Precision of the BKV Real-Time Quantitative PCR Assay

From: Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus

  

Crossing Point

  

BKV_5.1/Polyoma_4.2

BKV_4.1/Polyoma_4.2

BKV_4.1/BKV_2.1

Copies/Reaction*

# of Reactions

Mean

CV (%)

Std Dev

Mean

CV (%)

Std Dev

Mean

CV (%)

Std Dev

1.5 × 107

10

17.77

0.30

0.05

17.68

0.89

0.16

15.89

1.33

0.21

1.5 × 106

10

21.35

0.31

0.07

21.12

0.59

0.13

19.45

0.50

0.10

1.5 × 105

10

24.77

0.15

0.04

24.56

0.71

0.17

22.82

1.02

0.23

1.5 × 104

10

28.25

0.21

0.06

28.09

0.43

0.12

26.27

0.72

0.19

1.5 × 103

10

31.77

0.20

0.06

31.62

0.67

0.21

29.76

0.57

0.17

1.5 × 102

10

35.03

0.37

0.13

35.09

0.53

0.19

33.55

0.94

0.32

1.5 × 101

10

38.03

2.30

0.88

38.41

1.50

0.58

37.33

1.79

0.67

  1. *5 replicates of negative control were all negative
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Virology Journal

ISSN: 1743-422X