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Table 4 Precision of the BKV Real-Time Quantitative PCR Assay

From: Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus

   Crossing Point
   BKV_5.1/Polyoma_4.2 BKV_4.1/Polyoma_4.2 BKV_4.1/BKV_2.1
Copies/Reaction* # of Reactions Mean CV (%) Std Dev Mean CV (%) Std Dev Mean CV (%) Std Dev
1.5 × 107 10 17.77 0.30 0.05 17.68 0.89 0.16 15.89 1.33 0.21
1.5 × 106 10 21.35 0.31 0.07 21.12 0.59 0.13 19.45 0.50 0.10
1.5 × 105 10 24.77 0.15 0.04 24.56 0.71 0.17 22.82 1.02 0.23
1.5 × 104 10 28.25 0.21 0.06 28.09 0.43 0.12 26.27 0.72 0.19
1.5 × 103 10 31.77 0.20 0.06 31.62 0.67 0.21 29.76 0.57 0.17
1.5 × 102 10 35.03 0.37 0.13 35.09 0.53 0.19 33.55 0.94 0.32
1.5 × 101 10 38.03 2.30 0.88 38.41 1.50 0.58 37.33 1.79 0.67
  1. *5 replicates of negative control were all negative