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Table 3 Specificity of the BKV Real-Time Quantitative PCR Assay

From: Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus

    Crossing Point
    BKV_5.1/Polyoma_4.2
Copies BKV/Reaction* Copies JCV/Reaction* # of Reactions Mean Std Dev CV (%)
1.5 × 107 7.0 × 103 10 18.43 0.07 0.37
1.5 × 106 7.0 × 103 10 21.99 0.02 0.11
1.5 × 105 7.0 × 103 10 25.61 0.03 0.10
1.5 × 104 7.0 × 103 10 29.14 0.04 0.15
1.5 × 103 7.0 × 103 10 33.06 0.08 0.25
1.5 × 102 7.0 × 103 10 36.74 0.66 1.80
1.5 × 101 7.0 × 103 10 39.61 1.22 3.08
1.5 × 107 0 2 18.41 0.02 0.12
1.5 × 106 0 2 22.01 0.08 0.35
1.5 × 105 0 2 25.69 0.01 0.06
1.5 × 104 0 2 29.28 0.08 0.27
1.5 × 103 0 2 33.03 0.05 0.15
1.5 × 102 0 2 36.91 0.27 0.73
1.5 × 101 0 2 39.94 1.97 4.94
0 1.4 × 104 10 --- --- ---
Negative Control   5 --- --- ---