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Table 2 Sensitivity of the BKV Real-Time Quantitative PCR Assay

From: Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus

  

Crossing Point

  

BKV_5.1/Polyoma_4.2

BKV_4.1/Polyoma_4.2

BKV_4.1/BKV_2.1

Copies per Reaction*

# of Reactions

Mean

SD

CV

Mean

SD

CV

Mean

SD

CV

150

10

36.16

0.75

2.06

36.46

0.33

0.90

37.19

0.31

0.82

75

10

37.75

0.94

2.48

37.55

0.51

1.35

38.52

0.38

0.98

15

10

39.86

1.05

2.63

39.22

0.84

2.15

40.51

1.31

3.24

1.5**

10

40.84

0.58

1.42

41.08

0.22

0.53

43.75

2.17

4.96

  1. SD = standard deviation; CV is a percentage
  2. *5 replicates of negative control were all negative
  3. * *only 2 to 3 of 10 replicates for each primer combination had crossing points above background
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Virology Journal

ISSN: 1743-422X