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Table 2 Sensitivity of the BKV Real-Time Quantitative PCR Assay

From: Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus

   Crossing Point
   BKV_5.1/Polyoma_4.2 BKV_4.1/Polyoma_4.2 BKV_4.1/BKV_2.1
Copies per Reaction* # of Reactions Mean SD CV Mean SD CV Mean SD CV
150 10 36.16 0.75 2.06 36.46 0.33 0.90 37.19 0.31 0.82
75 10 37.75 0.94 2.48 37.55 0.51 1.35 38.52 0.38 0.98
15 10 39.86 1.05 2.63 39.22 0.84 2.15 40.51 1.31 3.24
1.5** 10 40.84 0.58 1.42 41.08 0.22 0.53 43.75 2.17 4.96
  1. SD = standard deviation; CV is a percentage
  2. *5 replicates of negative control were all negative
  3. * *only 2 to 3 of 10 replicates for each primer combination had crossing points above background