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Table 6 List of primers used for the deletion of ORF A and C

From: Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity

Primers

Sequence 5'-3'

1.8-kb mRNA(A+C)-kanR-F

5'-aaaggatctcaattaatagaacggcgattttttatttacggcgatatttg

CGTGTAGGCTGGAGCTGCTTCa-3'

1.8-kb mRNA(A+C)-kanR-R

5'-aaacagtttctaatcgaaagcgttaccgaacttgtctttaatgagaatcc

CATTCCGGGGATCCGTCGACa-3'

  1. aFor primers 1.8-kb(A+C)-kanR-F, 1.8-kb(A+C)-kanR-R, underlined sequences indicate the sequences from pKD13 used to amplify the KanR gene cassete with FRT, and sequences in bold indicate MDV sequence flanking the ORF (A+C).
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Virology Journal

ISSN: 1743-422X