Comparison of plaque characteristic of bac-GX0101Δ(A+C) and parental virus GX0101 in CEF. A: plaque of GX0101Δ(A+C); B: plaque of GX0101. GX0101 and GX0101Δ(A+C) were inoculated onto six-well plates seeded with CEFs and incubated at 37°C, 5% CO2, respectively. Visible viral plaques were confirmed by IFA with monoclonal antibody H19. The plaque size of GX0101Δ(A+C) was smaller than that of GX0101 at 96 h after infected in fresh CEF cells.