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Table 1 Primer and probe sequences for the duplex real-time RT-PCR for EEEV and WEEV

From: A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses

Name of primer or probe

Sequence (5'→3')

Nucleotide start

EEE-F

TGTGCGTACCTCCTCATCGTT

335

EEE-R

GACTGGCGTGAATCTCTGCTT

414

EEE-Probe

HEX-AGCAGCCTACCTTTCCGACAATGGTTGTC-TAMRA

364

WEE-F

AGGGATACCCCCGAAGGTT

8220

WEE-R

GTGAATAGCACACGGGTGGTT

8322

WEE- Probe

CTTTCGAATGTCACGTTCCCATGCG

8274