Comparison of ICC/q RT PCR cycle threshold (Ct) values of BGM monolayers infected with 10 PFU of poliovirus type 1 (strain LSc-1) to similarly infected BGM suspensions in 50 mL pp tubes on day 6. Each boxplot represents 3 trials run in triplicate. The average Ct values were 32.17 and 25.51 for the monolayers and suspensions respectively. One-way ANOVA was applied to interpret the Ct data. The groups were determined to be statistically significant different (p 0.000).