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Figure 8 | Virology Journal

Figure 8

From: MicroRNA profiling of tomato leaf curl new delhi virus (tolcndv) infected tomato leaves indicates that deregulation of mir159/319 and mir172 might be linked with leaf curl disease

Figure 8

Northern analysis of miR159 and miR172 at different days post inoculation (dpi) of ToLCNDV construct. a, Leaf samples obtained at 7, 14, 21, and 28 dpi of ToLCNDV agroinoculated tomato cv Pusa Ruby plants. Levels of miR159 and miR172 increase significantly as the ToLCNDV disease symptoms progress. The expression levels were normalized with respect to the ethidium bromide stained RNA bands. The calculated normalized levels are shown in parenthesis. b, The levels of miR159 were analyzed and found to induce in tomato ToLCNDV (2A+2B) infected cv JK Asha and chilli plants showing altered leaf morphology. 1: Healthy chilli leaves; 2: ToLCNDV (2A+2B) infected chilli leaves; 3: ToLCNDV (2A+2B) infected cv JK Asha leaves; 4: Healthy cv JK Asha. The EtBr stained gels serve as loading control.

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