Semi-quantitative Reverse transcription Polymerase chain reaction (RT-PCR) of various miRNA-targeted genes in (a) leaves and (b) flower tissues of different plants: healthy Pusa Ruby (lane 1), ToLCNDV (2A+2B) agroinfected Pusa Ruby (lane 2) and LA1777 (lane 3). The RT-PCR data of only few targets are displayed on the left of each panel and the fold changes of the individual targets as a result of virus infection were calculated (as mentioned above). The calculated changes have been shown on the right of each panel for a large number of targets. The band intensity of the targets was normalized with respect to actin amplification. The experiments were conducted twice.