Semi-quantitative reverse transcription Polymerase chain reaction (RT-PCR) of various pre-miRNAs in (A) leaves and (B) flower tissues of different plants viz ., Healthy Pusa Ruby (lane 1), ToLCNDV (2A+2B) agroinfected Pusa Ruby (lane 2) and LA1777 (lane 3). Upper panel shows pre-miR expression in leaf tissues and the lower panel corresponds to the flower tissues. The band intensity was normalized with respect to actin amplification. The fold change were calculated for ToLCNDV infected samples and is defined as ratio of normalized band intensity for ToLCNDV agroinfected sample (2) to that of healthy sample (1). The histograms were plotted as log2 (fold changes) for all the pre-miRNAs studied. The experiments were performed twice with significant reproducibility.