Figure 1From: Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon αThe different effect of HBc proteins on the MxA mRNA level in Huh7 cells was assessed by real time-PCR (a) and the level of Flag-tagged HBc protein was analyzed by western blot using an anti-Flag antibody (c). The different effect of HBc proteins on MxA promoter activity was also examined by luciferase assay (b) and the level of Flag-tagged HBc protein was analyzed by Western blot using an anti-Flag antibody (d). In the stably-transfected Huh7 cells, the expression of the WT and mutated (L60V, S87G and I97L) HBc proteins were detected by western blot using an anti-Flag antibody (e). In (c), (d), and (e), lanes 1-5 represent the expression of Flag-HBc proteins and β-actin in Huh7 cells transfected with pCMV-Tag1, pCMV-HBc (WT), pCMV-HBc (L60V), pCMV-HBc (S87G) and pCMV-HBc (I97L), respectively.Back to article page