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Figure 1 | Virology Journal

Figure 1

From: Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon α

Figure 1

The different effect of HBc proteins on the MxA mRNA level in Huh7 cells was assessed by real time-PCR (a) and the level of Flag-tagged HBc protein was analyzed by western blot using an anti-Flag antibody (c). The different effect of HBc proteins on MxA promoter activity was also examined by luciferase assay (b) and the level of Flag-tagged HBc protein was analyzed by Western blot using an anti-Flag antibody (d). In the stably-transfected Huh7 cells, the expression of the WT and mutated (L60V, S87G and I97L) HBc proteins were detected by western blot using an anti-Flag antibody (e). In (c), (d), and (e), lanes 1-5 represent the expression of Flag-HBc proteins and β-actin in Huh7 cells transfected with pCMV-Tag1, pCMV-HBc (WT), pCMV-HBc (L60V), pCMV-HBc (S87G) and pCMV-HBc (I97L), respectively.

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