Table 2 Ad35 viral vector packaging capacities.
From: Characterization of human adenovirus 35 and derivation of complex vectors
E1 deletion, transgene | Other genome modifications | pIX expression | Genome size (percent of wt) | Stability at Passage 3 |
|---|---|---|---|---|
d2, no transgene | None | No | 94.92 | Stable |
d2, green fluorescent protein (GFP) | None | No | 96.96 | Stable |
Δ447 - 3326, GFP | None | No | 97.22 | Stable |
d2, secretory alkaline phosphatase | None | No | 99.33 | Rearrangements |
d2, luciferase | None | No | 99.79 | Rearrangements |
d2, bacterial beta-glucuronidase | None | No | 99.94 | Rearrangements |
d2, HIV Env gp140ΔCFIΔV1V2 | None | No | 99.95 | Rearrangements |
d2, HIV Env gp140ΔCFI | None | No | 100.41 | Rearrangements |
d2, lacZ | None | No | 103.68 | Rearrangements |
d8, eGFP | E3(HE), E4(dORF3-6) | Yes | 85.60 | Stable |
d8, eGFP | E3(HE), E4(dAN) | Yes | 87.30 | Stable |
d5^, HIV Env gp140ΔCFIΔV1V2 | None | Yes | 100.43 | Stable |
d8, HIV Env gp140ΔCFI | None | Yes | 101.70 | Stable |
d6, Luciferase | None | Yes | 103.60 | Stable** |
d8, HIV Env gp140ΔCFI | Fiber modified* | Yes | 103.90 | Rearrangements |
d8, Luciferase | Fiber modified* | Yes | 104.11 | Rearrangements |