Table 1 Identification of Ad35 viral particle proteins.
From: Characterization of human adenovirus 35 and derivation of complex vectors
| Â | Protein mass spectroscopy molecular weight (MW) determination* | Â | Â | |||
|---|---|---|---|---|---|---|
| Â | Whole protein MW comparison | Tryptic peptide Identification | SDS-PAGE molecular weight** | |||
Protein | Experimental, theoretical (Da) | Δ (ppm) | No. of peptides | p-value | Identification | Apparent mass |
II (Hexon) | 107119.25, 107251.20 | 12.3 | n.d. | N/A | Size and abundance | 107 kDa |
II, n-term. fragment | n.d. | N/A | 4 | 8.50 × 10-13 | Tryptic peptide fingerprint | 16 kDa |
II, c-term. fragment | n.d. | N/A | n.d. | N/A | Abundance in RP--HPLC | 90 kDa |
III (Penton Base) | n.d., 62916.92 | N/A | 7 | 6.30 × 10-6 | SDS-PAGE of RP-HPLC fraction | 64.9 kDa |
IIIa | 64000.63, 63942.38 | 9.1 | 10 | 2.10 × 10-9 | SDS-PAGE of RP-HPLC fraction | 61 kDa |
IV (Fiber) | n.d., 35351.52 | N/A | n.d. | N/A | Size shift of modified protein | 35.3 kDa |
V | 40033.65, 40099.01 | 16.3 | 5 | 2.10 × 10-6 | Inference from MW | 46 kDa |
VI | 21783.45, 21728.76 | 25.2 | n.d | N/A | SDS-PAGE of RP-HPLC fraction | 28 kDa |
VII | 18784.53, 18738.47 | 24.6 | 3 | Manual analysis | Inference from MW, silver and Deep Purple staining intensity | 23.8 kDa |
VIII | 12204.10, 12190.81 | 10.9 | 5 | 1.50 × 10-9 | Inference from MW | 12 kDa |
VIII, c-term | 7644.21, 7697.31 | 69 | n.d. | N/A | N/A | N/A |
IX | 14149.42, 14193.94 | 31.4 | 2 | Manual analysis | Inference from MW and IX deficient virus | 14.1 kDa |