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Figure 6 | Virology Journal

Figure 6

From: Characterization of human adenovirus 35 and derivation of complex vectors

Figure 6

E4 transcriptional analysis. (A) E4 genomic and transcription map with probes used in northern blot analysis (not to scale). Genomic description is as in Figure 5 with the addition of putative splice donor (SD) and splice acceptor (SA) sites. The names of the identified RNAs are given to the right. ^ = splicing; An = polyadenylation.. Base pair coordinates of probes: ORF1 = 34,045-34,412, ORF2 = 33,611-34,015, ORF3 = 33,258-33,611, ORF4 = 32.882-33,214, ORF6 = 32,012-32,887, E4 = 31,855-34,550. (B) Northern blot analysis of RNA from Ad35 wild type-infected 293-ORF6 cells at 8 and 24 hpi. Probes are indicated above each lane and predicted transcripts are labeled on the left of the gel. Numbers on the right denote migration of RNA size standards. (C) Northern analysis at 24 hpi of RNA from wild type Ad35 with the following E4 regions: 1 = wt; 2 = AN; 3 = dORF6. Labeled as in panel B. Identity of transcript h was not determined.

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