Figure 5

Effect of E4 deletions on Ad35 growth. (A) Schematic of Ad35 E4 region and deletions (not to scale). The previously identified open reading frames 125R, 145R, 117R, 122R and 299R, are identified by their corresponding Ad5 open reading frame (ORF) ORF1, ORF2, ORF3, ORF4 and ORF6 respectively [6]. The Ad35 ORF6/7 has not been previously described and has coordinates 32,978-32,805 + 32082-31830. The name of the deletion is given to the right with the solid lines indicating which E4 sequences are retained. The description of the junctions are as follows: dORF6 32,010-32,877 with the stop codon of ORF4 changed to TAG, AN 32,007-33,083 with sequence CTAGTCTAGACTAG inserted, dORF3-6 32,010-33,604 with ORF2 stop codon changed to TAG, dORF1-2 33,604-34,416, dORF1-4 32,974-34,416, and ORF3 32,007-33,254 with sequence GCGCGTCGCGA inserted followed by 33,607-34,416. (B and C) Generation of viral progeny on the PC3 cell line. Presence (+) or absence (-) of ORF3 and ORF6 coding sequences is noted below each rAd. Active viral particles were determined at 72 hpi.