Figure 2
From: Characterization of human adenovirus 35 and derivation of complex vectors
Viral DNA synthesis. A549 cells were infected with wt Ad35 at an MOI of 5 focus forming units (FFU) per cell, the viral genome number at each time point was determined by qPCR with primers and probe to pIX coding sequences, standardized to total DNA, and expressed as viral genomes per ng of total DNA. Triplicate infections were performed for each time point; standard deviation error bars shown; hpi = hours post infection.