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Figure 1 | Virology Journal

Figure 1

From: Characterization of human adenovirus 35 and derivation of complex vectors

Figure 1

Identification of Ad35 viral particle proteins. (A) Representative reverse-phase HPLC chromatogram of Ad35 capsid proteins with identities shown. N-term II, C-term II = amino- and carboxy-terminal portions, respectively, of protein II. (B) Ad35 SDS-PAGE protein IV (fiber) identification. Ad35 viruses with wild type (WT) fiber or a genetically modified fiber protein containing the Ad5 fiber knob (5kIV) with predicted molecular masses of 35.4 and 33.9 kDa are indicated by an arrow and arrow head, respectively. Proteins were analyzed by SDS-PAGE and visualized by Deep Purple stain. Molecular weight markers are in the first lane along with their molecular weight in kDa. (C) SDS-PAGE protein assignments for Ad5 and Ad35 capsid proteins stained by silver. Purified virus was loaded at 2.5 × 1010 particles per lane. Molecular weight markers are in the first lane along with their molecular weight in kDa. Ad35 capsid proteins are annotated to the right.

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