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Figure 2 | Virology Journal

Figure 2

From: Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2

Figure 2

The expression of GST-ORF2-E protein was analyzed by SDS-PAGE (A) and Western-blotting (B) with an anti-GST monoclonal antibody. Lane 1, BL21 cell lysate before induction of IPTG; Lane 2, BL21 cell lysate after induction of IPTG; Lane 3, Supernatant of cell lysate after sonication and centrifugation; Lane 4, Pellet of cell lysate after sonication and centrifugation, There was a clear band of 29 kDa (arrow) after induction. The protein marker includes 8 bands at 175, 83, 62, 47.5, 32.5, 25, 16.5, and 6.5 kDa.

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Virology Journal

ISSN: 1743-422X