Detection of the BART miRNAs in total RNAs extracted from NPC xenografts and EBV-infected B-cells. Presence of BART miRNAs - miR-BART1-5p and 5 (cluster 1) and miR-BART 7-3p, 12 and 13 (cluster 2) - was assessed by real time PCR following multiplex RT-PCR. Abundance of each microRNA is assessed by 2-ΔCT calculation using the small cellular RNA RNU 44 as an endogenous reference. C15, C17 and C666-1 are NPC xenografts. CAPI is a xenografted EBV-negative epithelial tumor derived from a carcinoma of unknown primary. NAD+C15 is a lymphoblastoid cell line latently infected by an EBV isolate derived from the C15 NPC xenograft. Daudi is a Burkitt lymphoma cell line naturally infected by EBV and carrying its own distinct isolate. These data are representative of two similar experiments.