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Table 1 sequences and positions of designed siRNA

From: Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells

No

Sequence(5'-3')

Position

DenSi-1

AACGGAACCAGAUGACGUUGA

432

DenSi-2

AACUGUGCAUUGAAGCCAAAA

929

DenSi-3

AACAGGGCUAGACUUCAAUGA

1320

DenSi-4

AAGAAGAAUGGAGCGAUCAAA

133

control siRNA

UUCUCCGAACGUGUCACGUdT

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  1. Four siRNA sequences (table 1) against different parts of the DEN-1 genome were designed, the positions refered to DEN-1 reference strain (GenBank access No. EU848545). Negative siRNA was supplied by HiPerFect Transfection Reagent kit (Qiagen, German)