Susceptibility of turkeys to pandemic-H1N1 virus by reproductive tract insemination

Table 1 Results of qRRT-PCR testing for pH1N1 virus in oropharyngeal and cloacal swabs of experimental turkey hens inoculated intranasally, intracloacally, or intrauterine with A/Chile/3536/2009 (H1N1) virus.

Groups	Sampling day (days post inoculation) for swabs
	2		4		7		10		14
	OP^a	C^b	OP	C	OP	C	OP	C	OP	C
IN^c	0/8^d	0/8	0/7	0/7	0/7	0/7	0/6	0/6	0/6	0/6
IC^e	0/4	1/4(10^4.7)	0/4	0/4	0/3	0/3	0/2	0/2	0/2	0/2
IU^f	1/4 (10^4.7)^g	1/4(10^6.7)	3/3(10^4.7)	3/3(10^5.8)	0/3	3/3(10^5.7)	0/2	1/2(10^5.1)	1/2(10^4.8)	0/2

^a OP, oropharyngeal. ^b C, cloacal. ^c IN, intranasal. ^d number of virus positive/total sampled. ^e IC, intracloacal. ^f IU, intrauterine. ^g average titer of RNA positive samples. Previous studies have shown correlation between qRRT-PCR results and infectious titer of influenza A virus for oropharyngeal and cloacal swabs [15]. We report our qRRT-PCR data in relative equivalent units (REU) based on a standard curve for A/Chile/3536/2009 (H1N1) in mean chicken embryo infectious doses (EID₅₀)

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