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Figure 4 | Virology Journal

Figure 4

From: Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

Figure 4

ELISA monitoring immunoreactivity of salivary MUC5B and MUC7 mucins from HIV negative and positive individuals. Plate (A) was coated with MUC5B from HIV negative individual (dark blue diamond), MUC5B from HIV positive individuals with CD4 counts < 200 (pink square), 200-400 (blue triangle) and > 400 (pale blue cross) and plate (B) was coated with MUC7 from HIV negative individual (dark blue diamond), MUC7 from HIV positive individuals with CD4 counts < 200 (pink square), 200-400 (blue triangle) and > 400 (pale blue cross). Plates were incubated with serial two-fold dilutions of goat anti-MUC5B (A) and goat anti-MUC7 (B) polyclonal antibodies at concentrations between 8 μg/ml and 0.25 μg/ml (goat anti-MUC5B) and 12 μg/ml and 0.375 μg/ml (goat anti-MUC7). Antibody binding was detected using rabbit anti-goat HRPO linked secondary antibody and visualized with TMB/H2O2 substrate. Absorbance values were read at 405 nm in a Titertek ELISA reader. Each point is the average absorbance of duplicate samples. As a negative control wells were coated with PBS (+).

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