Figure 1

Anti-HIV-1 activities of salivary MUC5B and MUC7 of different CD4 counts for unfiltered samples. Salivary MUC5B and MUC7 mucins (500 μl or 0.9 mg each) from patients with CD4 count (< 200, 200-400 and > 400) were incubated with subtype D HIV-1 for 60 min and filtered through 0.45 μm pore size cellulose acetate filter. As controls HIV-1 treated with media and heat inactivated HIV-1 were used. The unfiltered samples were then incubated with CEM SS cells at a concentration of 0.5 × 10⁶cells/ml for 30 min, 1 h and 2 h. After PBS wash cells were cultured and viral replication was measured by a qualitative p24 antigen assay. Letters A, B and C indicate the anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with CD4 counts < 200, 200-400 and > 400 respectively.