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Figure 2 | Virology Journal

Figure 2

From: Interaction of human dipeptidyl peptidase IV and human immunodeficiency virus type-1 transcription transactivator in Sf9 cells

Figure 2

Co-immunoprecipitation of human-DPPIV and HIV1-Tat protein from Sf9 cells. Single expression or co-expression of Tat with DPPIV was performed for 72 hours, afterwards the cells were solubilised and lysates subjected to immunoprecipitation, (IP). (A) IP was conducted with anti-Tat mAb (lanes 2, 4 and 5) or with anti-DPPIV pAb (lanes 1, 3 and 7). Lane 6 was control conducted with protein-A-sepharose without antibody. Tat/vector represents control cells co-infected with Tat and a pFASTBAC1 vector virus. Each immunoprecipitate was separated by SDS-PAGE and the gels blotted and probed for with anti-Tat mAb. (B) IP was conducted with anti-Tat mAb (lanes 1 and 5) or with anti-DPPIV pAb (lanes 2 and 3) or with protein-A-sepharose without antibody (lane 4). The immunoprecipitates were subsequently blotted and probed for with anti-DPPIV pAb. The figure represents one of three independent experimental results.

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Virology Journal

ISSN: 1743-422X