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Figure 1 | Virology Journal

Figure 1

From: Interaction of human dipeptidyl peptidase IV and human immunodeficiency virus type-1 transcription transactivator in Sf9 cells

Figure 1

Co-localization of human-DPPIV and HIV1-Tat in Sf9 cells. Sf9 cells co-infected with Tat and DPPIV recombinant baculoviruses were harvested at the indicated time then fixed on culture slides and double-stained with the antibody combination: anti-Tat mAb/anti-mouse-Cy3 (red) and anti-DPPIV pAb/anti-rabbit-FITC (green). Nuclei were stained with DAPI (blue) and images were made at 100-fold magnification by confocal laser scanning microscopy. Co-localization of HIV1-Tat and human-DPPIV protein takes place at the cell membrane of merged images (white arrows).

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