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Figure 3 | Virology Journal

Figure 3

From: Intracellular expression of IRF9 Stat fusion protein overcomes the defective Jak-Stat signaling and inhibits HCV RNA replication

Figure 3

Phosphorylation of Stat1-GFP and Stat2-GFP fusion proteins in S9-13 and R15-3 cells determined by co-immunoprecipitation. S9-13 and R15-3 cells were transfected with either Stat1-GFP or Stat2-GFP. After 48 hours IFN-α (1,000 IU/ml) was added. Protein lysates were prepared, subjected to immunoprecipitation with a GFP antibody, and western blot analysis was performed using a p-STAT1 antibody.

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