Figure 6

MabJ2 detects HRV1A and 2 infections of diploid human lung airway cells. (A) Example images of WI-38 non-transformed primary human embryonic diploid airway cells inoculated with HRV1A or HRV2 and stained for dsRNA replication centers using mabJ2 (green) and nuclei (DAPI, blue) 7 h pi. Scale bar 100 μm. (B, C) WI-38 cells were inoculated with serial dilutions of HRV1A or HRV2 for 7 or 8 h at 33.5°C (blue) or 37°C (red), and infection was quantified by the mabJ2 dsRNA infection assay using automated image acquisition/analysis. The infection index is plotted in arbitrary units (AU), where 1 means all cells infected. (D) WI-38 cells were pre-treated with increasing concentrations of bafilomycin A1 (BafA1) for 30 min, and infected with HRV1A or HRV2 for 7 h. Quantification by the mabJ2 dsRNA infection assay was by automated image acquisition/analysis and the means (n = 3) and SEMs of the normalized infection index relative to DMSO carrier control infected cells are plotted.