Figure 4

Formation of dsRNA replication centers can be inhibited by pleconaril or guanidine-HCl. HeLa cells were either pre-incubated with different concentrations of pleconaril (A), or pleconaril [0.5 μg/ml] was added at indicated time points before or after infection (0 min) (B). The same types of experiments were done with guanidine-HCl (C, D guanidine HCl [2 mM]). Infections with indicated HRV or CV serotypes occurred at 37°C for 7 h, and were scored by automated analysis of mabJ2. Means and SEMs of one representative triplicate are shown.