Figure 3

Appearance of dsRNA replication centers is time, dose and temperature dependent and correlates with emergence of infectious titres. (A, B) The time and dose dependencies of HRV16 and CVB3 infections at 33.5°C (blue) or 37°C (red) were determined using the mabJ2 dsRNA infection assay in HeLa cells by either infection for 300 to 700 min, or with two fold serial dilutions of inocula. (C) To determine the correlation of mabJ2 dsRNA staining with viral titre production, HeLa cells were infected with HRV1A or 16 for 16 h (infection, blue) with serial dilutions of inocula. Newly synthesized particles were released from in parallel treated cells by three freeze/thaw cycles and inoculated on naïve HeLa cells to obtain single step growth curves (red). Infection was scored using automated image analysis. Means and SEMs of one representative triplicate are shown.