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Figure 5 | Virology Journal

Figure 5

From: Genome-wide analysis of host-chromosome binding sites for Epstein-Barr Virus Nuclear Antigen 1 (EBNA1)

Figure 5

Real-time PCR validation of ChIP-Seq data for EBNA1 binding sites near transcription starts. EBNA1 (black bars) or control IgG (grey bars) were assayed by ChIP in Raji cells for DNA at the EBV DS or cellular chromosome 11 cluster (A), the peaks found at the transcription start sites for CDC7, HDAC3, MAP3K7IP2, MAP3K1, IL6R, SIVA1, or negative control GAPDH (B), or EBNA1 peaks within genes for PARKIN, FOXP2, CDC6, SELK, NEK6, PITPNB, HFM1, EBV-OriLyt, JMJD2C, EEPD1, POU2F, CXCL13, DEK, PMF1, NRXN2, or DPM1/MOCS2. D) 293-EBV cells were transfected with FLAG-EBNA1 and assayed by ChIP with antibodies to FLAG (black bars) or IgG (grey bars) at the EBV DS, or cellular chromosome 11, CDC7, MAP3K1, IL6R, SIVA1, PARKIN, FOXP2, SELK, NEK6, PITPNB, HFM1, or negative controls for EBV-OriLyt, or cellular GAPDH.

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Virology Journal

ISSN: 1743-422X