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Figure 4 | Virology Journal

Figure 4

From: Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt

Figure 4

Diagnostic performance characteristics (field samples). Diagnostic performance characteristics of a newly developed H5-specific multiplex RT-qPCR for detection and differentiation of clade 2.2.1p proper (black triangles) and 2.2.1v variant HPAIV H5N1 strains (open triangles) from Egypt compared to standard H5 RT-qPCR [20]. The analysis comprises 50 M-PCR positive field samples obtained within the frame of the Egyptian poultry surveillance program from commercial poultry farms (n = 11) and backyard holdings (n = 39). See Additional file 4, Table S4, for individual values. Grey dots represent samples in which both clades were detected. X-axis: Ct-values obtained by the generic H5 RT-qPCR [20]. Y-axis: Ct-values generated by the specific component of the multiplex RT-qPCR as indicated above each graph.

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