Diagnostic performance characteristics (experimental infections). Diagnostic performance characteristics of a newly developed H5-specific multiplex RT-qPCR for detection and differentiation of clade 2.2.1p proper and 2.2.1v variant HPAIV H5N1 strains from Egypt compared to standard H5 RT-qPCR (Slomka et al. ). The analysis comprises swab samples obtained from SPF chickens after experimental infection with A/chicken/Egypt/0879-NLQP/2008 (clade 2.2.1v, open triangles, n = 34 samples) or A/chicken/Egypt/NLQP-0918Q/2009 (clade 2.2.1p, black triangles, n = 29 samples). See Additional file 3, Table S3, for individual values. Grey dots represent samples in which both clades were detected. X-axis: Ct-values obtained by the generic H5 RT-qPCR . Y-axis: Ct-values generated by the specific component of the multiplex RT-qPCR as indicated above each graph.