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Figure 2 | Virology Journal

Figure 2

From: Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt

Figure 2

Detection limits and amplification efficiency. Detection limits and amplification efficiency of the multiplex RT-qPCR for detection and differentiation of clade 2.2.1p proper and 2.2.1v variant HPAIV H5N1 strains from Egypt using cRNA as a copy-based target. The standard curves were established by up to five independent runs. Average Ct values and variations are plotted against the cRNA copy numbers.

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