Figure 2From: Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in EgyptDetection limits and amplification efficiency. Detection limits and amplification efficiency of the multiplex RT-qPCR for detection and differentiation of clade 2.2.1p proper and 2.2.1v variant HPAIV H5N1 strains from Egypt using cRNA as a copy-based target. The standard curves were established by up to five independent runs. Average Ct values and variations are plotted against the cRNA copy numbers.Back to article page