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Figure 1 | Virology Journal

Figure 1

From: Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt

Figure 1

Localization of primers and probes. Localization of primers and probes as mentioned in table 1 along the HA gene is depicted. Two target regions (P1, P2) were chosen. Primers and probes were selected with specificity for the HA of Egyptian HPAIV H5N1 viruses either of lineage 2.2.1p (proper) or 2.2.1v (variant). Triangles (2.2.1p) and squares (2.2.1v) distinguish nucleotide positions specific for either lineage. Labelling of hydrolysis probes was with FAM (F, 2.2.1v), HEX (H, 2.2.1p) or ROX (2.2.1p+v).

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Virology Journal

ISSN: 1743-422X