Characterization of an H3N2 triple reassortant influenza virus with a mutation at the receptor binding domain (D190A) that occurred upon virus transmission from turkeys to pigs
© Yassine et al; licensee BioMed Central Ltd. 2010
- Received: 2 August 2010
- Accepted: 30 September 2010
- Published: 30 September 2010
The hemagglutinin (HA) protein of influenza virus mediates essential viral functions including the binding to host receptor and virus entry. It also has the antigenic sites required for virus neutralization by host antibodies. Here, we characterized an H3N2 triple reassortant (TR) influenza virus (A/turkey/Ohio/313053/04) with a mutation at the receptor binding domain (Asp190Ala) that occurred upon virus transmission from turkeys to pigs in an experimental infection study. The mutant virus replicated less efficiently than the parental virus in human, pig and turkey primary tracheal/bronchial epithelial cells, with more than 3-log10 difference in virus titer at 72 hours post infection. In addition, the mutant virus demonstrated lower binding efficiency to plasma membrane preparations from all three cell types compared to the parental virus. Antisera raised against the parental virus reacted equally to both homologous and heterlogous viruses, however, antisera raised against the mutant virus showed 4-8 folds lower reactivity to the parental virus.
- Influenza Virus
- Receptor Binding Domain
- Avian Virus
- Plasma Membrane Preparation
Influenza A viruses infect a wide range of animal species including mammals and birds . All subtypes have been isolated from avian species, however, few subtypes have circulated and caused disease in mammals . Generally speaking, avian viruses preferentially bind to N-acetylneuraminic acid-α2,3-galactose form of sialic acid (α2,3-S.A.) receptors while human viruses preferentially bind to α2,6-S.A. receptors .
The HA is a major surface glycoprotein on influenza virus envelope and is essential for binding to host receptors and virus entry . In addition, it embraces the major immunogenic sites required for virus neutralization by host antibodies . Previous studies have identified key residues at the receptor binding domain (RBD) of the HA molecule that are critical in determining host range specificity of influenza viruses. In H2 and H3 subtypes, Gln226Leu and Gly228Ser mutations accounted for shifting from avian to human receptor binding specificity [6, 7]. In H1 subtypes, Glu190Asp and Gly225Glu mutations appear critical for adaptation of avian viruses to humans . Neither of the mutations observed in H1 or H3 viruses, that caused a shift from avian to human receptor binding specificity, correlated with the shift in binding specificity of H5 viruses .
In this study, we characterized an H3N2 triple reassortant (TR) influenza virus with a mutation at the RBD (Asp190Ala) that occurred upon virus transmission from turkeys to pigs in an experimental infection study . H3N2 TR viruses, which are characterized by having genes from human (HA, NA, and PB1), swine (NP, M, and NS) and avian (PB2, PA) lineage viruses, emerged in pigs in 1998 and then in turkeys in 2003 . The HA of H3N2 TR viruses is originally of human lineage viruses , and swine isolates of this subtype retain Asp at residue 190 of the RBD. Similarly, turkey isolates express Asp at the corresponding position, except for two isolates from Minnesota that expressed Val (NCBI gene bank accession number: ACF25543) or Ala (NCBI gene bank accession number: ACD35865) at the corresponding position.
In general, avian viruses express Glu (specific for α2,3-S.A. receptors) and human viruses expresses Asp (specific for α2,6 S.A. receptors) at position 190 of the RBD [8, 13]. Ala is rarely expressed at this position and characterization of such mutation is essential for its possible effect on antigenicity, receptor binding specificity, and interspecies transmission of H3 subtype influenza viruses [14–17].
Generation of mutant viruses
The H3N2 TR virus used in this study, A/turkey/Ohio/313053/04 (TK04), was previously isolated at our laboratory  and has been propagated two times in 10-day-old embryonated chicken eggs (ECE).
Utilizing the 12-plasmid reverse genetics system, we rescued the TK04 virus as previously described [18, 19]. Briefly, the HA, NP, NA, M, and NS genes were amplified with one-step RT-PCR kit (Qiagen, Valencia, CA), while the polymerase genes (PB1, PB2, and PA) were amplified with two-steps RT-PCR, using SuperscriptIII and Elongase Enzyme, respectively (Invitrogen, San Diego, CA). PCR products were purified and digested with Bsm BI restriction enzyme and cloned into pHH21 vector between promoter and terminator sequences of RNA polymerase I. Eight plasmids harboring the eight gene-segments were transfected along with four expression plasmids (pCAGGS-WSN-NP, pcDNA774-PB1, pcDNA762-PB2, and pcDNA787-PA, kindly provided by Dr. Y. Kawaoka, University of Wisconsin, Madison, WI) into 293T cells with the help of Lipofectamine-2000 reagent (Invitrogen, San Diego, CA). Supernatant from transfected cells was collected at 36 hours post transfection (hpi) and was subsequently inoculated into 10-day-old ECE for virus isolation. Single amino acid change at residue 190 of the RBD (Asp to Ala) was generated using QuikChange® Site-Directed Mutagenesis kit ( Stratagene, La Jolla, CA) based on manufacture protocol. In addition, we generated a virus with a mutation at residue 627 of PB2 gene (Glu627Lys) that has been shown to affect replication and transmission of influenza viruses in different species .
Assessment of virus replication in human, pig, and turkey tracheal/bronchial epithelial cells
Primary human tracheal/bronchial epithelial cells (HAEC) were purchased from Cell Application (Cell Application, San Diego, CA) and were maintained in tracheal/bronchial epithelial cells growth medium purchased from the same company (catalogue no. 511-500).
Primary pig and turkey tracheal/bronchial epithelial cells (PEC and TEC, respectively) were generated based on previously published protocols with slight modifications [21–23]. Briefly, distal-tracheal/proximal-primary bronchial airway tissues were collected from 5-weeks old healthy pig or 1-day old specific pathogen free (SPF) turkey. Tissues were cut into small fragments (~1 cm long) and were treated with pronase enzyme (1.4 mg/ml, Boehringer Mannheim, Indianapolis, IN) for 24-48 hours at 4°C. Pronase activity was stopped by adding 10% FBS in DMEM medium, cells were washed with PBS and then suspended in serum free mammary epithelial growth media supplemented with bovine pituitary extract, human epidermal growth factor, insulin and hydrocortisone (MEGM, Lonza, Walkersville, MD). To remove contaminating fibroblasts, cells were incubated for 2-4 hours at 37°C and 5% CO2 and non-adherent epithelial cells were collected and seeded into new culture flask for further growth. Cells were passaged up to five times prior to use in experiments.
For the kinetic study, 70-80% confluent cells seeded in 6-well plate were infected with either virus at 0.01 TCID50. Serum free DMEM media served as negative control. Plates were rocked every 15 minutes and inoculum was removed after 45 minutes followed by adding DMEM media supplemented with 1 μg/ml TPCK-treated trypsin on top of the cells. Supernatant from inoculated cells was collected at 24, 48, and 72 hpi and titrated in Madin-Darby canine kidney (MDCK) cells based on previously published protocol . Data were analyzed using graphPad prism software (GraphPad Software, Inc., La Jolla, CA, USA) by applying paired t-test with 95% confidence interval.
Assessment of cross reactivity between parental and mutant viruses
The cross hemagglutinin inhibition (HI) test was employed to evaluate the cross reactivity between parental (190Asp) and HA-mutant (190Ala) TK04 viruses. Additionally, cross reactivity was evaluated between TK04 parental and mutant viruses, and other H3N2 TR viruses isolated from turkeys in the United States (U.S.). This includes: A/turkey/North Carolina/03, A/turkey/Illinois/04, A/turkey/Minnesota/05, and A/turkey/North Carolina/05.
Antisera against TK04 viruses were produced by vaccinating two 2-week-old chickens with an inactivated virus vaccine (oil emulsion, 106 TCID50/ml) for three times in 2-weeks interval. HI test was carried out as previously described . Briefly, titers were determined by using two-fold serially diluted serum (25 μl), 4 HA units (25 μl) of homologous or heterologous antigen, and a 1% (50 μl) suspension of turkey erythrocyte per test well.
The antigenic relatedness between the different viruses was expressed as R-value based on the Archetti and Horsfall formula [12, 26]. The R-value is equivalent to the square root of r1 × r2, where r1 is the ratio of heterologous titer obtained with virus 2 to homologous titer obtained with virus 1; r2 is the ratio of the heterologous titer obtained with virus 1 to homologous titer obtained with virus 2.
Plasma membrane binding assay
Plasma membranes were prepared from HAEC, PEC, and TEC based on formerly published protocol [27–29]. Solid phase binding assay  was carried out as follows: plasma membrane preparations (PMP) were coated into 96-well plate (Costar, Lowell, MA) at concentration of 25 μg/ml overnight at 4°C. Plates were rinsed with PBS and then blocked with 0.2% BSA in PBS for 2 hours at 37°C. Two-fold serially diluted virus (50 μl; 64-4 HA) in reaction buffer (0.02% BSA in PBS) were added to wells and incubated at 4°C for one hour. Wells not coated with plasma membranes but blocked and treated with virus as indicated above were used as negative controls. Plates were then washed four times with ice-cold washing buffer (0.2XPBS containing 0.05% tween-80), followed by addition of 50 μl/well of peroxidase-labeled fetuin for 1 hour at 4°C. After four washes as indicated above, color was developed by adding 100 μl SureBlue TM-TMB substrate (KPL, Gaithersburg, MD) for 10 min at 37°C. The reaction was stopped with 100 μl 2N H2SO4 and OD450 nm measurement was obtained. Dose-response curves were generated by plotting OD450 nm values on y-axis and virus concentration (in HA units) on x-axis. To inhibit neuraminidase activity, all experiments were performed in the presence of Zanamivir hydrate (Moravek, CA, USA) at a final concentration of 0.25 μm. Recorded results are the average of three independent experiments.
In 1998, a new subtype of influenza A viruses, H3N2 TR, emerged in pig population in the U.S. and transmitted to other species including humans, turkeys, minks and waterfowls [11, 31–33]. In a previous study performed by our group, we evaluated the replication and transmission of H3N2 TR viruses between avian and mammalian species. Viruses that shared more than 99% of their genome sequences behaved differently in terms of transmission between swine and turkeys . Only one virus (A/turkey/Ohio/313053/04) transmitted efficiently both ways between swine and turkeys. Another virus (A/turkey/North Carolina/03) transmitted one way from pigs to turkeys but not vice verse. Neither of other two viruses (A/turkey/Illinois/04 and A/swine/North Carolina/03) transmitted either way between the two species. One of these viruses, TK04, which transmitted both ways between pigs and turkeys, expressed changes at or close to the RBD of the HA molecule upon transmission between the two species .
First, we evaluated the replication of TK04 parental and HA-mutant viruses (hereafter referred as 190Asp and 190Ala, respectively) in human, pig and turkey primary tracheal/bronchial epithelial cells. Virus with a mutation at residue 627 of the PB2 gene (Glu627Lys) was used as control, where such mutation has been shown to affect replication and host range specificity of influenza viruses.
Cross reactivity between TK04 parental (190Asp) and mutant (190Ala) viruses as well as other H3N2 TR viruses of turkey origin based on HI-test.
To further evaluate the above results, we included a wider range of turkey H3N2 TR viruses in the cross reactivity test. Again, anti-190Asp antisera reacted better against most turkey viruses compared to anti-190Ala antisera (Table 1). For example, Anti-190Asp showed similar reactivity to IL04 and homologous viruses, where both viruses share more than 98% of the HA protein sequences , including residue 190 of the RBD. However, Anti-190Ala exhibited four-fold lower reactivity to IL04 compared to the homologous virus. On the other hand, both antisera exhibited two-fold increase in reactivity to a 2005 strain from Minnesota (MN05) compared to homologous viruses. Interestingly, MN05 virus has been published to have similar mutation at residue 190 of the RBD (NCBI gene bank accession number: ACD35865), and thus, supporting the effect of such mutation on the antigenicity of H3N2 TR viruses.
Cross-HI results expressed as percent antigenic relatedness (R*)
Although antibodies to the HA-antigenic sites have been shown to affect receptor binding specificity and neutralization sensitivity, mutations solely to the RBD have not been shown to alter immunogenicity . In this paper, we report on naturally occurring mutation at the RBD of the HA molecule that affect antigenicity, binding efficiency, and replication competence of H3-subtype viruses.
Glu (specific for α2,3-S.A. receptors) is typically expressed in avian viruses at residue 190 of the HA molecule, while human viruses express Asp (specific for α2,6-S.A. receptors) at the corresponding position. Both amino acids are negatively charged, while Ala is a neutral amino acid. We assume that Ala at the corresponding position (Figure 1) might not affect the configuration, but rather the charge at RBD, explaining in part the above observed results. Hence, viruses with Ala at residue 190 of the RBD can survive in nature although with less fitness compared to 190Asp expressing viruses.
In conclusion, the Asp190Ala mutation that occurred upon virus transmission from turkeys to pigs could have been a transient or rare occurring mutation that resulted in a less fitted virus, explaining the rareness of Ala at this position in swine and turkey H3N2 influenza isolates. More work is needed to evaluate the replication and antigenicity of 190Ala mutation in vivo. Additionally, it is of importance to see the effect of the above mutation on the receptor binding specificity of H3 subtype viruses for its potential effect on interspecies transmission of influenza viruses.
This work was partially supported by funds from the United States Department of Agriculture, CSREES AI-CAP project, and the Ohio Agricultural Research and Development Center, The Ohio State University.
- Baigent SJ, McCauley JW: Influenza type A in humans, mammals and birds: determinants of virus virulence, host-range and interspecies transmission. Bioessays 2003, 25: 657-671. 10.1002/bies.10303PubMedView ArticleGoogle Scholar
- Palese P, Shaw M: Fields virology: Orthomyxoviridae, The Viruses and Their Replication. 5th edition. Philadelphia, PA: Lippincott Williams & Wilkins; 2007.Google Scholar
- Ito T: Interspecies transmission and receptor recognition of influenza A viruses. Microbiol Immunol 2000, 44: 423-430.PubMedView ArticleGoogle Scholar
- Skehel JJ, Wiley DC: Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. Annu Rev Biochem 2000, 69: 531-569. 10.1146/annurev.biochem.69.1.531PubMedView ArticleGoogle Scholar
- Schulze IT: Effects of glycosylation on the properties and functions of influenza virus hemagglutinin. J Infect Dis 1997,176(Suppl 1):S24-28. 10.1086/514170PubMedView ArticleGoogle Scholar
- Connor RJ, Kawaoka Y, Webster RG, Paulson JC: Receptor specificity in human, avian, and equine H2 and H3 influenza virus isolates. Virology 1994, 205: 17-23. 10.1006/viro.1994.1615PubMedView ArticleGoogle Scholar
- Rogers GN, Paulson JC, Daniels RS, Skehel JJ, Wilson IA, Wiley DC: Single amino acid substitutions in influenza haemagglutinin change receptor binding specificity. Nature 1983, 304: 76-78. 10.1038/304076a0PubMedView ArticleGoogle Scholar
- Matrosovich M, Tuzikov A, Bovin N, Gambaryan A, Klimov A, Castrucci MR, Donatelli I, Kawaoka Y: Early alterations of the receptor-binding properties of H1, H2, and H3 avian influenza virus hemagglutinins after their introduction into mammals. J Virol 2000, 74: 8502-8512. 10.1128/JVI.74.18.8502-8512.2000PubMedPubMed CentralView ArticleGoogle Scholar
- Stevens J, Blixt O, Tumpey TM, Taubenberger JK, Paulson JC, Wilson IA: Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus. Science 2006, 312: 404-410. 10.1126/science.1124513PubMedView ArticleGoogle Scholar
- Yassine HM, Al-Natour MQ, Lee CW, Saif YM: Interspecies and intraspecies transmission of triple reassortant H3N2 influenza A viruses. Virol J 2007, 4: 129. 10.1186/1743-422X-4-129PubMedPubMed CentralView ArticleGoogle Scholar
- Tang Y, Lee CW, Zhang Y, Senne DA, Dearth R, Byrum B, Perez DR, Suarez DL, Saif YM: Isolation and characterization of H3N2 influenza A virus from turkeys. Avian Dis 2005, 49: 207-213. 10.1637/7288-101304RPubMedView ArticleGoogle Scholar
- Yassine HM, Lee CW, Suarez DL, Saif YM: Genetic and antigenic relatedness of H3 subtype influenza A viruses isolated from avian and mammalian species. Vaccine 2008, 26: 966-977. 10.1016/j.vaccine.2007.11.094PubMedView ArticleGoogle Scholar
- Nobusawa E, Ishihara H, Morishita T, Sato K, Nakajima K: Change in receptor-binding specificity of recent human influenza A viruses (H3N2): a single amino acid change in hemagglutinin altered its recognition of sialyloligosaccharides. Virology 2000, 278: 587-596. 10.1006/viro.2000.0679PubMedView ArticleGoogle Scholar
- Tumpey TM, Maines TR, Van Hoeven N, Glaser L, Solorzano A, Pappas C, Cox NJ, Swayne DE, Palese P, Katz JM, Garcia-Sastre A: A two-amino acid change in the hemagglutinin of the 1918 influenza virus abolishes transmission. Science 2007, 315: 655-659. 10.1126/science.1136212PubMedView ArticleGoogle Scholar
- Gamblin SJ, Haire LF, Russell RJ, Stevens DJ, Xiao B, Ha Y, Vasisht N, Steinhauer DA, Daniels RS, Elliot A, et al.: The structure and receptor binding properties of the 1918 influenza hemagglutinin. Science 2004, 303: 1838-1842. 10.1126/science.1093155PubMedView ArticleGoogle Scholar
- Yang ZY, Wei CJ, Kong WP, Wu L, Xu L, Smith DF, Nabel GJ: Immunization by avian H5 influenza hemagglutinin mutants with altered receptor binding specificity. Science 2007, 317: 825-828. 10.1126/science.1135165PubMedPubMed CentralView ArticleGoogle Scholar
- Glaser L, Stevens J, Zamarin D, Wilson IA, Garcia-Sastre A, Tumpey TM, Basler CF, Taubenberger JK, Palese P: A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity. J Virol 2005, 79: 11533-11536. 10.1128/JVI.79.17.11533-11536.2005PubMedPubMed CentralView ArticleGoogle Scholar
- Lee CW, Senne DA, Suarez DL: Generation of reassortant influenza vaccines by reverse genetics that allows utilization of a DIVA (Differentiating Infected from Vaccinated Animals) strategy for the control of avian influenza. Vaccine 2004, 22: 3175-3181. 10.1016/j.vaccine.2004.01.055PubMedView ArticleGoogle Scholar
- Neumann G, Watanabe T, Ito H, Watanabe S, Goto H, Gao P, Hughes M, Perez DR, Donis R, Hoffmann E, et al.: Generation of influenza A viruses entirely from cloned cDNAs. Proc Natl Acad Sci USA 1999, 96: 9345-9350. 10.1073/pnas.96.16.9345PubMedPubMed CentralView ArticleGoogle Scholar
- Steel J, Lowen AC, Mubareka S, Palese P: Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N. PLoS Pathog 2009, 5: e1000252. 10.1371/journal.ppat.1000252PubMedPubMed CentralView ArticleGoogle Scholar
- Busch MG, Bateman AC, Landolt GA, Karasin AI, Brockman-Schneider RA, Gern JE, Suresh M, Olsen CW: Identification of amino acids in the HA of H3 influenza viruses that determine infectivity levels in primary swine respiratory epithelial cells. Virus Res 2008, 133: 269-279. 10.1016/j.virusres.2008.01.014PubMedView ArticleGoogle Scholar
- Zaffuto KM, Estevez CN, Afonso CL: Primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses. Avian Pathol 2008, 37: 25-31. 10.1080/03079450701774850PubMedView ArticleGoogle Scholar
- Kong BW, Foster LK, Foster DN: Comparison of avian cell substrates for propagating subtype C avian metapneumovirus. Virus Res 2006, 116: 58-68. 10.1016/j.virusres.2005.08.013PubMedView ArticleGoogle Scholar
- Reed L, Muench H: A simple method of estimating fifty per cent end-points. Am J Hyg 1938., 27:Google Scholar
- Purchase HG: A Laboratory manual for the isolation and identification of avian pathogens. Kennett Square, PA Kendall/Hunt Pub Co.; 1989.Google Scholar
- Archetti I, Horsfall FL Jr: Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum. J Exp Med 1950, 92: 441-462. 10.1084/jem.92.5.441PubMedPubMed CentralView ArticleGoogle Scholar
- Gambaryan A, Webster R, Matrosovich M: Differences between influenza virus receptors on target cells of duck and chicken. Arch Virol 2002, 147: 1197-1208. 10.1007/s00705-002-0796-4PubMedView ArticleGoogle Scholar
- Gambaryan AS, Matrosovich MN: A solid-phase enzyme-linked assay for influenza virus receptor-binding activity. J Virol Methods 1992, 39: 111-123. 10.1016/0166-0934(92)90130-6PubMedView ArticleGoogle Scholar
- Gambaryan AS, Marinina VP, Tuzikov AB, Bovin NV, Rudneva IA, Sinitsyn BV, Shilov AA, Matrosovich MN: Effects of host-dependent glycosylation of hemagglutinin on receptor-binding properties on H1N1 human influenza A virus grown in MDCK cells and in embryonated eggs. Virology 1998, 247: 170-177. 10.1006/viro.1998.9224PubMedView ArticleGoogle Scholar
- Wan H, Perez DR: Quail carry sialic acid receptors compatible with binding of avian and human influenza viruses. Virology 2006, 346: 278-286. 10.1016/j.virol.2005.10.035PubMedView ArticleGoogle Scholar
- Webby RJ, Swenson SL, Krauss SL, Gerrish PJ, Goyal SM, Webster RG: Evolution of swine H3N2 influenza viruses in the United States. J Virol 2000, 74: 8243-8251. 10.1128/JVI.74.18.8243-8251.2000PubMedPubMed CentralView ArticleGoogle Scholar
- Gagnon CA, Spearman G, Hamel A, Godson DL, Fortin A, Fontaine G, Tremblay D: Characterization of a Canadian mink H3N2 influenza A virus isolate genetically related to triple reassortant swine influenza virus. J Clin Microbiol 2009, 47: 796-799. 10.1128/JCM.01228-08PubMedPubMed CentralView ArticleGoogle Scholar
- Ramakrishnan MA, Wang P, Abin M, Yang M, Goyal SM, Gramer MR, Redig P, Fuhrman MW, Sreevatsan S: Triple reassortant swine influenza A (H3N2) virus in waterfowl. Emerg Infect Dis 16: 728-730.Google Scholar
- Zhu H, Wang J, Wang P, Song W, Zheng Z, Chen R, Guo K, Zhang T, Peiris JS, Chen H, Guan Y: Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice. Virology 401: 1-5. 10.1016/j.virol.2010.02.024Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.