Double-stranded siRNA could effectively inhibit NCAM expression in N2a cells. N2a cells were transfected with siRNA targeted against NCAM. The cells were harvested after siRNA transfection and analyzed by FACS with rabbit anti-NCAM antibody and FITC-conjugated goat anti-rabbit IgG (H+L). Mock-transfected siRNA N2a cells served as a control. There appeared to be a slight decrease of the positive rate of N2a KD cells compared to that of controls within 72 hours.