Figure 3

Western blot analysis of proteins expression in total extracts of 293T cells transfected with the pcDNA3.1 (+) expression plasmid. Lane 1: Western blot analysis of NCAM protein expression. The full lengths cDNA of NCAM gene was used to construct the transfect plasmid. Cell lysates from 293T cells were run on a 10% SDS-PAGE gel and blotted onto polyvinylidene difluoride membranes. The blots were probed with a 1:10 dilution of the rabbit anti-NCAM polyclonal IgG (200 μg/ml). The antibodies were detected by horseradish peroxidaseconjugated goat anti-rabbit IgG antibodies and chemiluminescence. Lane 2: Immunoblots for Hdac2 protein. The blot was probed with a 1:10 dilution of the rabbit anti-Hdac2 polyclonal IgG (200 μg/ml). The antibodies were detected by horseradish peroxidaseconjugated goat anti-rabbit IgG antibodies. Lane 3: Immunoblots for RPS13 protein. The blot was probed with a 1:10 dilution of the goat anti-RPS13 polyclonal IgG (200 μg/ml). The antibodies were detected by horseradish peroxidaseconjugated mouse anti-goat IgG antibodies. Lane 4: Immunoblots for Sf3b2 protein. The blot was probed with a 1:5 dilution of the mouse monoclonal anti-Sf3b2 IgG2a (100 μg/ml). The antibodies were detected by horseradish peroxidaseconjugated goat anti-mouse IgG antibodies. Lane 5: The 293T cells transfected with vector alone.