Figure 1
From: Identification of NCAM that interacts with the PHE-CoV spike protein
The results of display of the cDNA library from N2a cells on T7 phage. (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified mRNA of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained >90% recombinants with average insert size of >300 bp. M: DL2000 Marker.