Table 2 Intra- and inter-assay co-efficient of variation for EBNA-1 and BHRF-1 QPCRs.
DNA Target (copies/5 ul) | Mean CT | Mean R-G 6000™ Results (copies/5ul) | Standard Deviation of R-G 6000™ Results (copies/5ul) | Mean % Variation | COV (%) | Mean R2 |
|---|---|---|---|---|---|---|
EBNA-1 Intra-Assay Variation (same day) | ||||||
100,000 | 18.03 | 87,329 | 6,670 | 12.68% | 7.64 | 0.991 |
10,000 | 21.28 | 11,735 | 3,092 | 26.30% | 26.34 | |
1,000 | 25.21 | 1,057 | 100 | 7.00% | 9.50 | |
100 | 29.10 | 103 | 38 | 32.12% | 37.25 | |
10 | 32.91 | 11 | 6 | 46.16% | 57.50 | |
EBNA-1 Intra-Assay Variation (different days) | ||||||
100,000 | 16.94 | 89,643 | 8,164 | 11.00% | 9.11 | 0.998 |
10,000 | 20.31 | 10,678 | 1,207 | 10.00% | 11.31 | |
1,000 | 23.85 | 1,133 | 129 | 16.00% | 11.41 | |
100 | 27.65 | 102 | 4 | 3.00% | 3.90 | |
10 | 31.42 | 10 | 2 | 17.88% | 18.95 | |
BHRF-1 Intra-Assay Variation (same day) | ||||||
100,000 | 17.23 | 97,884 | 9,144 | 8.08% | 9.34 | 0.994 |
10,000 | 20.91 | 9,852 | 542 | 4.45% | 5.50 | |
1,000 | 24.38 | 1,146 | 202 | 16.12% | 17.64 | |
100 | 28.43 | 94 | 19 | 17.70% | 20.51 | |
10 | 31.95 | 11 | 5 | 35.38% | 41.91 | |
BHRF-1 Intra-Assay Variation (different days) | ||||||
100,000 | 18.05 | 105,387 | 4,621 | 6.02% | 4.38 | 0.997 |
10,000 | 21.75 | 9,779 | 818 | 6.23% | 8.37 | |
1,000 | 25.23 | 1,042 | 141 | 11.63% | 13.55 | |
100 | 29.06 | 89 | 18 | 13.76% | 19.88 | |
10 | 32.66 | 12 | 2 | 25.30 | 16.53 | |
EBNA-1 Inter-Assay Variation | ||||||
100,000 | 19.87 | 101,644 | 14,058 | 10.99% | 28.35 | 0.990 |
10,000 | 23.75 | 10,660 | 1,471 | 13.65% | 13.80 | |
1,000 | 27.68 | 1,084 | 191 | 18.67% | 17.60 | |
100 | 31.71 | 111 | 49 | 33.58% | 43.75 | |
10 | 35.86 | 12 | 9 | 65.03% | 75.85 | |
BHRF-1 Inter-Assay Variation | ||||||
100,000 | 17.30 | 109,065 | 14,266 | 10.01% | 13.08 | 0.990 |
10,000 | 21.49 | 9,209 | 2,154 | 16.84% | 23.39 | |
1,000 | 25.49 | 860 | 251 | 22.19% | 29.15 | |
100 | 29.01 | 108 | 49 | 35.53% | 45.33 | |
10 | 32.29 | 15 | 8 | 69.41% | 57.23 | |