Skip to main content

Table 2 Intra- and inter-assay co-efficient of variation for EBNA-1 and BHRF-1 QPCRs.

From: Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye

DNA Target
(copies/5 ul)
Mean CT Mean R-G 6000™ Results
(copies/5ul)
Standard Deviation of R-G 6000™ Results
(copies/5ul)
Mean % Variation COV
(%)
Mean R2
EBNA-1 Intra-Assay Variation (same day)
100,000 18.03 87,329 6,670 12.68% 7.64 0.991
10,000 21.28 11,735 3,092 26.30% 26.34  
1,000 25.21 1,057 100 7.00% 9.50  
100 29.10 103 38 32.12% 37.25  
10 32.91 11 6 46.16% 57.50  
EBNA-1 Intra-Assay Variation (different days)
100,000 16.94 89,643 8,164 11.00% 9.11 0.998
10,000 20.31 10,678 1,207 10.00% 11.31  
1,000 23.85 1,133 129 16.00% 11.41  
100 27.65 102 4 3.00% 3.90  
10 31.42 10 2 17.88% 18.95  
BHRF-1 Intra-Assay Variation (same day)
100,000 17.23 97,884 9,144 8.08% 9.34 0.994
10,000 20.91 9,852 542 4.45% 5.50  
1,000 24.38 1,146 202 16.12% 17.64  
100 28.43 94 19 17.70% 20.51  
10 31.95 11 5 35.38% 41.91  
BHRF-1 Intra-Assay Variation (different days)
100,000 18.05 105,387 4,621 6.02% 4.38 0.997
10,000 21.75 9,779 818 6.23% 8.37  
1,000 25.23 1,042 141 11.63% 13.55  
100 29.06 89 18 13.76% 19.88  
10 32.66 12 2 25.30 16.53  
EBNA-1 Inter-Assay Variation
100,000 19.87 101,644 14,058 10.99% 28.35 0.990
10,000 23.75 10,660 1,471 13.65% 13.80  
1,000 27.68 1,084 191 18.67% 17.60  
100 31.71 111 49 33.58% 43.75  
10 35.86 12 9 65.03% 75.85  
BHRF-1 Inter-Assay Variation
100,000 17.30 109,065 14,266 10.01% 13.08 0.990
10,000 21.49 9,209 2,154 16.84% 23.39  
1,000 25.49 860 251 22.19% 29.15  
100 29.01 108 49 35.53% 45.33  
10 32.29 15 8 69.41% 57.23  
  1. Abbreviations: CT, cycle threshold; Mean % variation, average percentage variation between the calculated (Rotor-Gene results) and the given concentration (DNA target); COV, co-efficient of variation is the ratio of standard deviation to the mean; R2-value, square root of the correlation co-efficient - in quantitation PCR describes the percentage of the data which matches the hypothesis that the standards conform to a line of best fit.