Table 1 Primers and probe used to detect KIPyV, WUPy and MCPyV.
From: Newly discovered KI, WU, and Merkel cell polyomaviruses: No evidence of mother-to-fetus transmission
PCR and Virus | Primer Sequence 5 → 3 | Region | Amplicon size | |
|---|---|---|---|---|
Forward | Reverse | |||
Nested PCR outer primers | ||||
KIPyV, WUPyV | ATCTRTAGCTGGAGGAGCAGAG | CCYTGGGGATTGTATCCTGMGG | VP2 | 336 bp |
MCPyV (LT3) | TTGTCTCGCCAGCATTGTAG | ATATAGGGGCCTCGTCAACC | LT | 309 bp |
MCPyV (LT1) | TACAAGCACTCCACCAAAGC | TCCAATTACAGCTGGCCTCT | LT | 440 bp |
Nested PCR inner primers | ||||
KIPyV, WUPyV | RTCAATTGCTGGWTCTGGAGCTGC | TCCACTTGSACTTCCTGTTGGG | VP2 | 277 bp |
MCPyV (LT3) | TGACGTGGGGAGAGTGTTTTTG | GAGGAAGGAAGTAGGAGTCTAGAAAAG | LT | 155 bp |
MCPyV (M1) | GGCATGCCTGTGAATTAGGA | TTGCAGTAATTTGTAAGGGGACT | LT | 179 bp |
MCPyV qPCR primers | TGCCTCCCACATCTGCAAT | GTGTCTCTGCCAATGCTAAATGA | VP1 | 59 bp |
MCPyV probe | FAM-TGTCACAGGTAATATC-MGBNFQ |