From: Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease
RNA strand
Slope
Reaction Efficiency (%)
R2
Specificity (% non-specific strand detected)
Negative (genomic)
Y = -3.44x+41.11
95
0.997
< 0.001
Positive (mRNA and RI)
Y = -3.37x+41.06
98