Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease

Table 2 Standard curve fits, R² and specificity of the QPCR.

RNA strand	Slope	Reaction Efficiency (%)	R²	Specificity (% non-specific strand detected)
Negative (genomic)	Y = -3.44x+41.11	95	0.997	< 0.001
Positive (mRNA and RI)	Y = -3.37x+41.06	98	0.997	< 0.001

Specificity was assessed by spiking non strand-specific RNA standards into naïve mouse lung RNA. Detection of spiked non strand-specific RNA is expressed as a percentage of measured specific sense standards spiked to mouse lung. Reaction Efficiency defined as 10^(-1/slope) - 1.

ISSN: 1743-422X