Table 1 Primer sequences used for RNA standard generation, cDNA synthesis and QPCR.

In vitro standard external positive sense

TCCAGCAAATACACCATCCA

In vitro standard external negative sense

CTGCTTCACCACCCAATTTT

In vitro standard nested positive sense

ATAGAATTC GGTATGTTATATGCGATGTCTAGGT¹

In vitro standard nested positive sense

ATAGGATCC TGCTAAGACTCCCCACCGTAA²

Positive sense RNA-specific cDNA synthesis

CGGTCATGGTGGCGAATAA TCCTGCAAAAATCCCTTCAACT³

Negative sense RNA-specific cDNA synthesis

CGGTCATGGTGGCGAATAA ACTTTATAGATGTTTTTGTTCA³

Positive sense-specific QPCR primer

CCCCACTTTATAGATGTTTTTGTTCA

Negative sense-specific QPCR primer

TCCTGCAAAAATCCCTTCAACT

QPCR tag primer

CGGTCATGGTGGCGAATAA

Probe

FAM-TTGGTATAGCACAATCTTCTACCAGAGGTGGC-TAMRA