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Figure 2 | Virology Journal

Figure 2

From: Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease

Figure 2

RSV infection and replication in BALB/c mouse lungs. Mice were dosed with either 1 × 106 FFU RSV A2 or an equivalent concentration of UV-inactivated RSV. Three mice per treatment were sampled at 1, 5, 8, 24, 48 and 72 hours and after 7, 10, 37 and 59 days post-infection. One lung per animal was processed for QPCR analyses, the other for infectivity assay. A) Levels of positive and negative sense RSV RNA in mouse lungs were monitored using strand-specific QPCR. Normalised RSV copy number was determined from strand-specific RNA standard curves corrected by beta actin arbitrary copy number. Means ± SEM for 3 animals per time point are plotted. Lower limit of detection = 80 actin normalised copies per gram lung wt. B) Infectious live virus in mouse lungs was monitored by FFU assay (lower limit of detection = 102 FFU/g lung wt.) up to day 7 post infection. Individual measurements are plotted and bars indicate mean values.

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Virology Journal

ISSN: 1743-422X