Figure 1From: Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus diseaseAn RSV strand-specific QPCR method. Strand-specific priming was performed during cDNA synthesis and QPCR was performed using a primer/probe set designed to amplify part of the nucleocapsid region. A) Negative sense RNA standard curve. B) Positive sense strand RNA standard curve. Duplicate measurements are plotted.Back to article page