Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes

Table 1 Identification of PVM isolates in potato samples*

Isolates	Potato Cultivar	Origin	Symptoms	ELISA^a Test 1/Test 2	PCR/RFLP^b
Isolates
CL1	Shepody	OLF	Mosaic	+/+	+/+
CL2	RB	OLF	Mosaic	+/+	+/+
CL3	GM	OLF	Mosaic	+/+	+/+
CL4	A82705-1	UI	Mosaic	+/+	+/+
Ca5	Shepody	PEI	Mosaic	+/+	+/+
Ca99	Shepody	PEI	Mosaic	+/+	+/+
Ca102	Shepody	PEI	Mosaic	+/+	+/+
Ca128	Shepody	PEI	Mosaic	+/+	+/+
Ca414	Shepody	PEI	Mosaic	+/+	+/+
Ca508	Shepody	PEI	Mosaic	+/+	+/+
Ca513	Shepody	PEI	Mosaic	+/+	+/+
Controls
Negative	Shepody	CL	Healthy	-/-	-/-
NTC	N/A	N/A	N/A	-/-	-/-

*CL: Canadian Food Inspection Agency (CFIA) - Charlottetown Laboratory; OLF: CFIA - Ottawa Laboratory (Fellow Field); UI: University of Idaho (Idaho, USA); PEI: the province of Prince Edward Island; RB: Russet Burbank; GM: Green Mountain; NTC: no template control; N/A: not applicable; +: positive results; -: negative results.
a. The results are shown as initial test of samples/confirmatory test of inoculated plants (positive threshold: 0.1).
b. The results are shown as PCR amplification/RFLP confirmation using Msc I.

ISSN: 1743-422X