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Figure 5 | Virology Journal

Figure 5

From: Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections

Figure 5

Finer mapping the epitope M14-13. Peptide M14 was truncated from the carboxy and amino terminal. After short protein fragments were fusion expressed with GST, they were analyzed by ELIZA using MAb 2F7. When 2 or more amino acid residues were removed from the carboxy terminal and 4 or more amino acid residues were removed form the amino terminal, the reactivity between MAb 2F7 and peptide fusion protein decreased greatly. This result shows that peptide M14-13 (KEAWLDSTKAT) is the minimal requirement for MAb 2F7 to recognize the linearized epitope.

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Virology Journal

ISSN: 1743-422X