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Figure 2 | Virology Journal

Figure 2

From: Quantitative analysis of interferon alpha receptor subunit 1 and suppressor of cytokine signaling 1 gene transcription in blood cells of patients with chronic hepatitis C

Figure 2

Validation of socs1 real-time RT-PCR. A. Socs1 detection assay was based on the use of Sybr Green. Validation curves were performed with RNA extracted from HeLa cells, and the threshold cycle (ΔCT) difference between socs1 and hprt was calculated and drawn with respect to RNA concentration. Slope value (y) indicates that hprt can be used for relative quantification of socs1 transcription. B. Assay specificity was corroborated by means of the dissociation curves. Peaks correspond to the melting temperatures for socs1 and hprt genes.

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Virology Journal

ISSN: 1743-422X