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Figure 1 | Virology Journal

Figure 1

From: Quantitative analysis of interferon alpha receptor subunit 1 and suppressor of cytokine signaling 1 gene transcription in blood cells of patients with chronic hepatitis C

Figure 1

Validation of ifnar1 real-time RT-PCR. Ifnar1 detection assay was based on the use of TaqMan probes. Validation curves were performed with RNA extracted from HeLa cells, and the threshold cycle difference (ΔCT) between ifnar1 and the constitutive endogenous gene hprt was calculated and drawn with respect to RNA concentration. Slope value (y) indicates that hprt can be used for relative quantification of ifnar1 transcription.

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Virology Journal

ISSN: 1743-422X