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Table 2 Cellular proteins identified in purified PRRSV virions by 2-DE PAGE and MALDI-TOF/TOF MS

From: Profiling of cellular proteins in porcine reproductive and respiratory syndrome virus virions by proteomics analysis

Spot no. a

Protein name b

Accession no. c

Protein Score d

Protein MW(kDa) e

Protein pI f

Pep Count g

Intensity Matched h

Subcellular Location i

Cytoskeletal Proteins

7

keratin 10

gi|21961605

124/60

58.79

5.09

24

22.125

INF

12

coronin, actin binding protein, 1B

gi|197100107

414/338

55.68

5.96

18

38.007

C, CYS

27,29,40

keratin 9

gi|55956899

150/97

62.03

5.14

14

18.608

INF

69,101

        

39

tubulin, beta polypeptide

gi|57209813

821/618

47.74

4.7

27

77.274

MIT, C

41

alpha-tubulin

gi|37492

679/534

50.13

5.02

23

72.834

MIT

42,43

tubulin, alpha, ubiquitous

gi|77539752

349/236

50.10

4.98

19

37.589

MIT

51,52,95

Beta-actin

gi|40744574

604/482

41.71

5.37

24

76.044

C, CYS

53

actin, gamma 1 propeptide

gi|4501887

797/602

41.77

5.31

26

77.03

C, CYS

58

keratin 1

gi|11935049

537/472

66.03

8.16

18

12.481

INF

82

tropomyosin 1 alpha chain isoform 4

gi|63252900

493/295

32.86

4.72

26

55.547

C, CYS

102

cofilin 1 (non-muscle)

gi|5031635

269/197

18.49

8.22

10

29.949

N, C, CYS

Stress proteins

6

Heat shock 70 kDa protein 8 isoform 1

gi|5729877

938/675

70.85

5.37

38

72.617

C

24

Heat shock 60 kDa protein 1

gi|31542947

521/364

61.02

5.7

28

53.064

C

75

ribosomal protein P0

gi|4506667

572/462

34.25

5.71

14

41.767

C

94,98,99

Heat shock protein 27

gi|662841

449/339

22.31

7.83

14

57.414

C, N

Metabolism-associated proteins

17

transketolase

gi|388891

495/382

67.84

7.89

16

57.92

CYO

18

pyruvate kinase

gi|35505

806/486

57.84

7.58

41

69.771

C, CYO

32

phosphoglycerate dehydrogenase

gi|23308577

526/397

56.61

6.29

23

42.346

C

33

aldehyde dehydrogenase 1A1

gi|21361176

601/461

54.83

6.3

22

51.887

C, CYO

34

UDP-glucose dehydrogenase

gi|4507813

600/350

54.99

6.73

33

72.634

C

49,50

enolase 1

gi|4503571

813/609

47.14

7.01

28

49.053

C

62

phosphoglycerate kinase 1A isoform 2

gi|4505763

738/553

44.59

8.3

26

53.6

C

71,72

glyceraldehyde-3-phosphate dehydrogenase

gi|37730278

654/543

23.85

9.17

13

38.336

C

73,84

guanine nucleotide binding protein (G protein), beta polypeptide 1

gi|197100735

253/176

37.35

5.47

15

27.248

ISPM

74

L-lactate dehydrogenase B

gi|4557032

493/335

36.62

5.71

18

64.612

C

78

Chain A, Fidarestat Bound To Human Aldose Reductase

gi|13096112

194/122

35.70

6.56

12

24.868

C

81

PREDICTED: lactate dehydrogenase

gi|109107094

457/283

36.61

8.45

24

56.639

C

96

peroxiredoxin 1

gi|4505591

433/305

22.10

8.27

16

54.055

C

97

proteasome activator hPA28 suunit beta

gi|1008915

271/191

27.33

5.44

13

29.728

CYO

100

triosephosphate isomerase 1

gi|4507645

677/547

26.65

6.45

17

54.228

CYO

Macromolecular biosynthesis

14

chaperonin containing TCP1, subunit 3 (gamma)

gi|14124984

356/221

60.36

6.1

20

43.055

C

15

chaperonin containing TCP1, subunit 6A (zeta 1)

gi|197099952

541/389

58.04

6.3

23

55.446

C

26

chaperonin containing TCP1, subunit 5 (epsilon) protein

gi|24307939

700/424

59.63

5.45

40

64.77

C, N

30

chaperonin containing TCP1, subunit 2

gi|5453603

799/500

57.45

6.01

38

64.220

C, CYO

31

PRP19/PSO4 pre-mRNA processing factor 19 homolog

gi|7657381

425/304

55.15

6.14

21

31.179

N

37

retinoblastoma binding protein 4 isoform a

gi|5032027

277/202

47.63

4.74

13

41.617

N

54

eukaryotic translation initiation factor 4A isoform 1

gi|4503529

731/530

46.12

5.32

29

64.901

CYO

86

proliferating cell nuclear antigen

gi|49456555

513/400

28.69

4.57

16

36.423

N

Glycoprotein

35,36

alpha2-HS glycoprotein

gi|2521981

73/51

35.64

5.2

10

10.222

S

Calcium-regulated membrane-binding protein

79,80

Annexin A2

gi|18645167

769/568

38.55

7.57

28

83.608

S, EXS

83

Annexin A5

gi|4502107

234/122

35.91

4.94

13

39.159

C

85

Annexin A4

gi|4502105

640/465

36.06

5.84

26

78.847

C

104

S100 calcium binding protein A10

gi|4506761

275/210

11.20

6.82

6

23.65

MIT

Regulate apoptosis

103

galectin-1

gi|4504981

257/236

14.7

5.25

12

20.78

C, S

Others

13

T-complex protein 1 isoform a

gi|57863257

602/396

60.31

5.8

31

55.471

M

64,70

gastric-associated differentially-expressed proteinYA61P

gi|6970062

305/262

14.86

6.84

7

13.425

?

  1. a Protein spot numbers on 2-DE gel.
  2. b Alternative names are provided in parentheses.
  3. c Accession numbers ford NCBI database (accessible at http://www.ncbi.nlm.nih.gov/).
  4. d MASCOT protein score (based on combined MS and MS/MS spectra) of greater than 64 (p≤0.05) or the total ion score (based on MS/MS spectra) of greater than 30 (p≤0.05)
  5. e Theoretical molecular mass.
  6. f Theoretical pI.
  7. g Observed peptides that differ either by sequence, modification or charge.
  8. h Sequence coverge is based on peptides with unique sequence.
  9. i The proteins subcellular location. INF-Intermediate filament; C-Cytoplasm; CYS-Cytoskeleton; N-Nucleus; CYO-Cytosol; ISPM-Internal side of plasma membrane;S- Secreted; EXS- Extracellular space; MIT- Mitochondrion; M- Membrane; ?-unknown
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Virology Journal

ISSN: 1743-422X