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Table 2 Cellular proteins identified in purified PRRSV virions by 2-DE PAGE and MALDI-TOF/TOF MS

From: Profiling of cellular proteins in porcine reproductive and respiratory syndrome virus virions by proteomics analysis

Spot no. a Protein name b Accession no. c Protein Score d Protein MW(kDa) e Protein pI f Pep Count g Intensity Matched h Subcellular Location i
Cytoskeletal Proteins
7 keratin 10 gi|21961605 124/60 58.79 5.09 24 22.125 INF
12 coronin, actin binding protein, 1B gi|197100107 414/338 55.68 5.96 18 38.007 C, CYS
27,29,40 keratin 9 gi|55956899 150/97 62.03 5.14 14 18.608 INF
69,101         
39 tubulin, beta polypeptide gi|57209813 821/618 47.74 4.7 27 77.274 MIT, C
41 alpha-tubulin gi|37492 679/534 50.13 5.02 23 72.834 MIT
42,43 tubulin, alpha, ubiquitous gi|77539752 349/236 50.10 4.98 19 37.589 MIT
51,52,95 Beta-actin gi|40744574 604/482 41.71 5.37 24 76.044 C, CYS
53 actin, gamma 1 propeptide gi|4501887 797/602 41.77 5.31 26 77.03 C, CYS
58 keratin 1 gi|11935049 537/472 66.03 8.16 18 12.481 INF
82 tropomyosin 1 alpha chain isoform 4 gi|63252900 493/295 32.86 4.72 26 55.547 C, CYS
102 cofilin 1 (non-muscle) gi|5031635 269/197 18.49 8.22 10 29.949 N, C, CYS
Stress proteins
6 Heat shock 70 kDa protein 8 isoform 1 gi|5729877 938/675 70.85 5.37 38 72.617 C
24 Heat shock 60 kDa protein 1 gi|31542947 521/364 61.02 5.7 28 53.064 C
75 ribosomal protein P0 gi|4506667 572/462 34.25 5.71 14 41.767 C
94,98,99 Heat shock protein 27 gi|662841 449/339 22.31 7.83 14 57.414 C, N
Metabolism-associated proteins
17 transketolase gi|388891 495/382 67.84 7.89 16 57.92 CYO
18 pyruvate kinase gi|35505 806/486 57.84 7.58 41 69.771 C, CYO
32 phosphoglycerate dehydrogenase gi|23308577 526/397 56.61 6.29 23 42.346 C
33 aldehyde dehydrogenase 1A1 gi|21361176 601/461 54.83 6.3 22 51.887 C, CYO
34 UDP-glucose dehydrogenase gi|4507813 600/350 54.99 6.73 33 72.634 C
49,50 enolase 1 gi|4503571 813/609 47.14 7.01 28 49.053 C
62 phosphoglycerate kinase 1A isoform 2 gi|4505763 738/553 44.59 8.3 26 53.6 C
71,72 glyceraldehyde-3-phosphate dehydrogenase gi|37730278 654/543 23.85 9.17 13 38.336 C
73,84 guanine nucleotide binding protein (G protein), beta polypeptide 1 gi|197100735 253/176 37.35 5.47 15 27.248 ISPM
74 L-lactate dehydrogenase B gi|4557032 493/335 36.62 5.71 18 64.612 C
78 Chain A, Fidarestat Bound To Human Aldose Reductase gi|13096112 194/122 35.70 6.56 12 24.868 C
81 PREDICTED: lactate dehydrogenase gi|109107094 457/283 36.61 8.45 24 56.639 C
96 peroxiredoxin 1 gi|4505591 433/305 22.10 8.27 16 54.055 C
97 proteasome activator hPA28 suunit beta gi|1008915 271/191 27.33 5.44 13 29.728 CYO
100 triosephosphate isomerase 1 gi|4507645 677/547 26.65 6.45 17 54.228 CYO
Macromolecular biosynthesis
14 chaperonin containing TCP1, subunit 3 (gamma) gi|14124984 356/221 60.36 6.1 20 43.055 C
15 chaperonin containing TCP1, subunit 6A (zeta 1) gi|197099952 541/389 58.04 6.3 23 55.446 C
26 chaperonin containing TCP1, subunit 5 (epsilon) protein gi|24307939 700/424 59.63 5.45 40 64.77 C, N
30 chaperonin containing TCP1, subunit 2 gi|5453603 799/500 57.45 6.01 38 64.220 C, CYO
31 PRP19/PSO4 pre-mRNA processing factor 19 homolog gi|7657381 425/304 55.15 6.14 21 31.179 N
37 retinoblastoma binding protein 4 isoform a gi|5032027 277/202 47.63 4.74 13 41.617 N
54 eukaryotic translation initiation factor 4A isoform 1 gi|4503529 731/530 46.12 5.32 29 64.901 CYO
86 proliferating cell nuclear antigen gi|49456555 513/400 28.69 4.57 16 36.423 N
Glycoprotein
35,36 alpha2-HS glycoprotein gi|2521981 73/51 35.64 5.2 10 10.222 S
Calcium-regulated membrane-binding protein
79,80 Annexin A2 gi|18645167 769/568 38.55 7.57 28 83.608 S, EXS
83 Annexin A5 gi|4502107 234/122 35.91 4.94 13 39.159 C
85 Annexin A4 gi|4502105 640/465 36.06 5.84 26 78.847 C
104 S100 calcium binding protein A10 gi|4506761 275/210 11.20 6.82 6 23.65 MIT
Regulate apoptosis
103 galectin-1 gi|4504981 257/236 14.7 5.25 12 20.78 C, S
Others
13 T-complex protein 1 isoform a gi|57863257 602/396 60.31 5.8 31 55.471 M
64,70 gastric-associated differentially-expressed proteinYA61P gi|6970062 305/262 14.86 6.84 7 13.425 ?
  1. a Protein spot numbers on 2-DE gel.
  2. b Alternative names are provided in parentheses.
  3. c Accession numbers ford NCBI database (accessible at http://www.ncbi.nlm.nih.gov/).
  4. d MASCOT protein score (based on combined MS and MS/MS spectra) of greater than 64 (p≤0.05) or the total ion score (based on MS/MS spectra) of greater than 30 (p≤0.05)
  5. e Theoretical molecular mass.
  6. f Theoretical pI.
  7. g Observed peptides that differ either by sequence, modification or charge.
  8. h Sequence coverge is based on peptides with unique sequence.
  9. i The proteins subcellular location. INF-Intermediate filament; C-Cytoplasm; CYS-Cytoskeleton; N-Nucleus; CYO-Cytosol; ISPM-Internal side of plasma membrane;S- Secreted; EXS- Extracellular space; MIT- Mitochondrion; M- Membrane; ?-unknown